Abstract
Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti-P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B, iNOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti-P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and iNOS2 of neuron cells.
Highlights
Any reports and responses or comments on the article can be found at the end of the article
This study aims to evaluate the effect of anti-P. gingivalis antibodies on TNF, IL1B, and inducible nitric oxide synthase (iNOS) gene expression when bacteria interact with neuron cells
From qPCR analysis, it was observed that there are differences in the gene expression of TNF-α, IL-1β and iNOS in SHSY-5Y cells that have been exposed to P. gingivalis with and without antibody coating, it can be concluded that the research hypothesis is accepted
Summary
Any reports and responses or comments on the article can be found at the end of the article. It is likely that if we observed using a viability test such as the MTT test (MTT 3- (4,5-dimethyltiazol-2-yl) -2,5diphenyltetrazolium bromide), the cell count would be able to be counted. Another possibility is that the number of cells did not differ, but the cell’s metabolism changed between experimental groups, characterized by differences in the mRNA expression of neuroinflammatory cytokine. The limitation of using 96 well plate culture is the cell number in each well is very limited. Using 24 well plate cultures will get more appropriate RNA samples to be analysed in order to overcome this problem and the statistical analysis will be employed
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