Abstract
Bladder cancer (BC) is one of the tumors which occur most frequently in urological system, but less is known about the expression of tight junction proteins and its clinical significance in BC. In this study, expression of claudin-4, zonula occludens-1 (ZO-1) and zonula occludens-1 nucleic acid-binding protein (ZONAB), in BC tissues, adjacent nontumor tissue (ANTT), and BC cell lines was examined by Western blotting, semiquantitative RT-PCR, and immunohistochemistry, and then, the clinical significance of these proteins was investigated. The mRNA and protein expression of ZONAB were significantly upregulated, while those of ZO-1 was significantly downregulated in some BC cell lines and tissues in comparison with nontumor urothelial cell lines and ANTT. High expression rate of ZO-1 and ZONAB had negative correlation in BC tissues and was also correlated with muscle-invasive lesions in BC tissues. In conclusion, the expression of tight junction proteins is significantly altered in BC and ZO-1, and ZONAB interaction might be involved in BC development.
Highlights
Bladder cancer (BC) is one of the tumors which occur most frequently in the urological system
Claudin-4, zonula occludens-1 (ZO-1), and zonula occludens-1 nucleic acid-binding protein (ZONAB) mRNA expression was detected in sv-huc-1, 5637, UMUC-3, and T24 cell lines through real-time PCR to verify those findings from Western blotting
We observed the change of tight junction protein expression in BC
Summary
Bladder cancer (BC) is one of the tumors which occur most frequently in the urological system. Intercellular adhesion is the first barrier against development of solid tumors [2]. Tight junction is a protein strand that seals the gap between adjacent cells and separates the lateral intercellular space from apical lumen of the epithelium [5]. It participates in intercellular signaling and involves in several biological processes [6,7,8]. Tight junctions consist of three groups of proteins: integral membrane proteins, plaque anchoring proteins, and tight junction regulatory proteins [9, 10]. The integrity of tight junctional component is vital for the maintenance of cell polarity, morphology, and barrier function [10, 14, 15]
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