Abstract
Aspergillus fumigatus (A. fumigatus) is an opportunistic fungus that causes invasive aspergillosis. Determining the immune changes during A. fumigatus infection and the factors leading to such changes clearly will make it possible to prevent the spread of the infection and to provide new strategies in the treatment of infection. Thus, the present study aims at determining the changes of lymphocyte surface antigens which develop during A. fumigatus infection and the role of cytokines in immune response. The expression of the surface antigens of lymphocytes was analysed by flow cytometry and the cytokine levels were determined by ELISA in a mouse model of aspergillosis. It was observed that in mice infected by A. fumigatus the percentage of CD19+ B cells and the levels of IL-4 and IL-10 increased when compared to those in noninfected mice cells. These results suggest that Th2 type cytokines are important in the pathogenesis of A. fumigatus infection. Humoral immunity is considered to be effective during A. fumigatus infection because of the increase in Th2 type response.
Highlights
Aspergillus fumigatus (A. fumigatus), the most common cause of invasive aspergillosis, is an opportunistic fungus
We did not find a significant difference between the CD4+ lymphocyte and CD8+ T lymphocyte percentages of the mononuclear cells in mice infected with A. fumigatus and those of the control groups without A. fumigatus infection (p>0.05)
Surface expression of CD25 (IL-2R), normally increased on activated T lymphocytes, was not found to have increased in the specimens with A. fumigatus infection in 24 hours compared to the control groups
Summary
Aspergillus fumigatus (A. fumigatus), the most common cause of invasive aspergillosis, is an opportunistic fungus. Cell-mediated immunity and humoral immunity are the principle protective immune responses against fungal infections. Specific antibodies occur during fungal infections but the role of antibodies in protective immunity is unclear [3]. Aspergillus fumigatus (A. fumigatus) is an opportunistic fungus that causes invasive aspergillosis. The present study aims at determining the changes of lymphocyte surface antigens which develop during A. fumigatus infection and the role of cytokines in immune response. Methodology: The expression of the surface antigens of lymphocytes was analysed by flow cytometry and the cytokine levels were determined by ELISA in a mouse model of aspergillosis. Humoral immunity is considered to be effective during A. fumigatus infection because of the increase in Th2 type response.
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