Abstract
ABSTRACT Objective: Therefore, the purpose of the currently study was to analyze the expression of the STRO-1 stem cell marker and the marker related to Hsp25 differentiation and dental development during odontogenesis in rats. Methods: Eighteen-day Wistar rat embryos and 7-day-old animals were analyzed morphologically via Hematoxylin and Eosin (HE) staining and via immunohistochemical marking for Stro-1 and Hsp25 in 5uM serial sections. Results: In the HE morphological analysis in 18-day embryos, the stage of tooth development was in the cap phase, while in the 7-day old animals it was in the bell phase. Conclusion: The expression of STRO-1 in the embryos was not found in the region of the dental papilla or enamel organ, however, in the 7-day-old animals, STRO-1-positive cells were found in the region of the dental follicle and dental papilla. As for the Hsp25, this exhibited weak marking in the cap phase while in the bell phase, there was a reaction, mainly in the odontoblasts. The expressions in the site of these markers vary according to the stage of tooth development.
Highlights
The major differentiation potential of dental stem cells lies in the formation of dentin and tissue associated with the periodontium, regardless of whether these cells are derived from the pulp, apical papilla, periodontal ligament or dental follicle [1,2,3]
There was joint expression of STRO-1 with dentinogenic markers such as bone sialophosphoprotein, osteocalcin and FGFR1 growth factors (Fibroblast Growth Factor Receptor 1) and TGFßRI (Transforming Growth Factor-ß Receptor Type 1). These findings suggest that the marked cells are of primary odontoblast origin that form root dentin
In the 18-day-old embryos, the tooth development stage was in the bell phase
Summary
In 2003, Shi & Gronthos [5] isolated human stem cells from the bone marrow and dental pulp through the STRO-1, a specific mesenchymal stem-cell marker, and analyzed the expression of antigens in the endothelial cells, smooth muscle pericytes. For Huang et al [8], this line of evidence suggests that cells in the apical papilla derive from developing tissue which represents a young stem-cell population, in other words, a superior source of cells for the regeneration of tissue. This demonstrates the important aspect that the developing tissue may contain stem cells different from the already formed tissue
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