Expression of the genes of non-specific immune response in the body of gallus gallus domesticus under the influence of immunodepressive vaccine viruses

Abstract

Relevance. The body reacts to the impact of infectious agents by activating the processes of gene expression in cells and tissues of various organs, which results in the formation of messenger RNA, complementary to the DNA of the expressed genes. This article discusses the effect of vaccine viruses of infectious bursal disease and infectious anemia of chickens on the morphology of target organs of the immune system of chickens, as well as the expression of a number of key genes of nonspecific immune response in the cells of these organs.Methods. As an example of a vaccine virus for infectious bursal disease, an immunocomplex vaccine against infectious bursal disease from the VNIVIP strain was taken; as a sample of a vaccine virus for infectious anemia in chickens, the Nobilis® CAV P4 vaccine manufactured by MSD Animal Health was taken.Results. It was concluded that vaccine viruses come into contact with target organs (the bursa of Fabricius and thymus), causing a number of pathological changes. The expression of the immune genes IL8L2, PTGS2, IRF7 was also determined as the main genes responsible for the synthesis of antiviral and inflammatory proteins. Based on the results of the studies, it was concluded that the IL8L2 and PTGS2 genes responsible for the synthesis of inflammatory components are actively expressed when the cells are exposed to the viruses of infectious anemia of chickens and infectious bursal disease. The expression of the IRF7 gene in the immune organs of the experimental groups of chickens did not practically differ from the level of expression in the target organs of the control birds due to the immunosuppressive effect of the viruses.