Expression of the alpha1-alpha6 collagen IV chains in the dermoepidermal junction during human foetal skin development: temporal and spatial expression of the alpha4 collagen IV chain in an early stage of development.

Abstract

To study the expression of the alpha1-alpha6 chains of type IV collagen in the dermoepidermal junction (DEJ) during human foetal skin development, human foetal (10 and 20 weeks of gestation) and adult skin was immunostained with specific monoclonal antibodies to the alpha1-alpha6 chains of type IV collagen. Intense expression of the alpha4 chain and weak expression of the alpha2 and alpha6 chains were observed in the DEJ of 10 weeks gestational skin. In contrast, the alpha1, alpha2, alpha5 and alpha6 chains were detected in the DEJ of 20 weeks gestational and adult skin. Preferential expression of alpha4 during the early phase of development (10 weeks of gestation) may suggest a chain-specific regulatory mechanism for type IV collagen expression and its potential role in DEJ formation during development.