Expression of testis specific expressed gene 2 in murine testicular torsion/detorsion model

Abstract

Objective To explore the expression profiles of testis specific expressed gene 2 (TSEG-2)in murine testicular torsion/detorsion(TD)model.Methods Thirty six Kunming mice were randomly divided into control(n=6),sham(n=6),and experimental(n=24)groups.In the experimental group,the left testes of the TD mice were rotated 720 degrees for 2 hrs,and then detorsed.The testes were harvested after 1 and 7 days of detorsion.H&E staining and TUNEL staining were performed for morphological and apoptotic studies.The activities of superoxide disnmtase(SOD)and malondialdehycle(MDA)were measured by xanthine superoxidase and thiobarbiuric acid,respectively.The TSEG-2 expression in testes was detected by in situ hybridization and real-time quantitative PCR.Results Compared with the serniniferous tubules of the control and sham operated mice,the sparse structure and apoptotic spermatogenic cells of the seminiferous tubules significantly increased in the mice subjected to TD.One day or 7 days after TD,the Johnsen's score was reduced by 23.4% and 64.1%,respectively(P<0.01).The SOD activities were decreased by 11.6%and 22.2%,respectively(P<0.05).The MDA increased by 69.6%and 93.2%,respectively(P<0.01).The positive staining for TSEG-2 mRNA localized at the spermatogonia and spermatocytes of seminiferous tubules.One day or 7 days after TD,the TSEG-2 expression was enhanced by 2.2 and 6.6 folds,respectively.Conclusions The enhanced TSEG-2 expression may be correlated with impaired anti-oxidative activities and incrassated apoptosis of spermatogenic cells in the murine testicular torsion/detorsion model. Key words: Testcular torsion; Testis specific expressed gene 2; Models,animal