Expression of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain on CD4+T cells and regulatory T cells in recurrent spontaneous abortion patients

Abstract

Objective To evaluate the correlation of T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain (TIGIT) on peripheral CD4+T cells and regulatory T (Treg) cells with recurrent spontaneous abortion (RSA). Methods Totally 60 RSA patients(RSA group) and 68 normal pregnant women (control group) were collected in Obstetrics and Gynecology Hospital of Fudan University during July 2017 to August 2018. Flow cytometry was used to detect the proportion of TIGIT+T cells on CD4+T cells and Treg cells. Results Compared with normal pregnant women, the proportion of CD4+T and TIGIT+CD4+T cells in RSA patients were not different, the proportion of Treg cells was lower in RSA group (5.08%±1.94%) than in control group (3.15%±1.16%) (P=0.02), but the proportions of TIGIT+FoxP3+CD4+T in RSA group (56.41%±12.36%) was higher than that in control group (47.82%±10.53%) (P=0.03). Conclusion The abnormal expression of TIGIT on Treg cells in RSA patients, may be related to the occurrence of RSA, which may be a new therapeutic target for RSA. Key words: Recurrent spontaneous abortion; Regulatory T cell; T cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain; CD4+T cell