Expression of synaptophysin and its mRNA in bovine corpus lutea during different stages of pregnancy

Abstract

In order to investigate the expression of mRNA and protein for synaptophysin (SYP) in bovine corpus luteum (CL) during different stages of pregnancy, we chose Holstein cows during various pregnancy stages. The CL was divided into two parts, then immunohistochemical streptavidin-perosidase and RT-PCR were used to determine the levels of protein and mRNA for SYP respectively. SYP immunoreactive products mainly located in large luteal cells; much less or no immunoreactivity was found in small luteal cells. The expression levels of SYP were different in various stages of pregnancy. In the CL of mid pregnancy, the levels of protein and mRNA for SYP were both significantly higher than those in early and late stage of pregnancy (P<0.05). After parturition, compared with late stage of pregnancy, the protein level of SYP decreased (P<0.05), but its mRNA increased (P<0.05). In conclusion, SYP has the strongest expression in mid stage of pregnancy, and its regular expression in bovine CL indicates that SYP may play important roles in maintaining the function of bovine CL and in the regulation of production.