Expression of suppressor of cytokine signaling 3 in liver and spleen of septic mice

Abstract

Objective To investigate the expression change of suppressor of cytokine signaling 3 (SOCS3) in the liver and the spleen of septic mice, and to find out its possible mechanism in sepsis. Methods Cecal ligation and puncture (CLP) was adopted to make the sepsis model in mice. The liver and the spleen tissue were taken to detect the mRNA and protein expression of SOCS3. The level of SOCS3 mRNA expression was detected by RT-PCR, and the level of SOCS3 protein expression was examined by Western blot. SPSS statistics software was used to analysis the correlation between the expression of SOCS3 mRNA and protein. Results The gene and protein expression of SOCS3 only showed a trend of up-regulation in the liver after CLP, and there was no difference comparing with the control group (P>0.05). The expressions ot SOCS3 gene and protein in the spleen were obviously higher than those in the control group at 2 h and reached the peak at 12 h after operation. Positive correlation was found between gene and protein expression in the liver and spleen(r=0.353, 0.731, all P<0.05). Conclusions CLP-induced sepsis can up-regulate the expression of SOCS3 in mice, indicating that SOCS3may play an important role in the change of immune system in sepsis. Key words: Sepsis; Suppressor of cytokine signaling 3 ; Gene expression; Liver; Spleen; Mice