Expression of specificity protein 1 and collagen I in primary pterygial tissues.

Bo Jiang,Yong Wang,Zhang-You Wu,Zi-Cheng Zhu

doi:10.5935/0004-2749.20190074

Abstract

To determine the expression profiles of the transcription factor specificity protein 1 and collagen I in primary pterygial and normal conjunctival tissues, and to explore the role of specificity protein 1 and collagen I in pterygial development. The pterygial tissues of 20 patients who underwent resection of primary pterygial tissue in our hospital from June 2016 to December 2017 and the conjunctival tissues of 10 patients with enucleation due to trauma were collected. Reverse transcription quantitative-po lymerase chain reaction and western blot analyses were used to detect the relative expression levels of specificity protein 1 and type I collagen at the mRNA and protein levels. The content of specificity protein 1 and collagen I mRNA and protein was significantly greater in primary pterygial tissue than it was in conjunctival tissue (p<0.05). There was a positive correlation between the mRNA and protein levels of specificity protein 1 and collagen I in primary pterygial tissues (protein: r=1, p<0.05; mRNA: r=1, p<0.05). Specificity protein 1 and collagen I are expressed in normal conjunctival and pterygial tissues, but expression is significantly greater in the latter. Specificity protein 1 and collagen I may be involved in the regulation of the development of primary pterygium.

Highlights

Introduction[1,2] Histological studies have found that pterygium is composed of atrophic conjunctival epithelium, hypertrophic vascularized tissue, and degenerated connective tissue, while ultrastruc-
Protein expression levels of specificity protein 1 (Sp1) and collagen I were significantly greater in the pterygial tissues than they were in the conjunctival tissues (p
MRNA expression levels of Sp1 and collagen I were significantly greater in pterygial tissues than they were in the conjunctival tissues (p

Summary

Introduction

[1,2] Histological studies have found that pterygium is composed of atrophic conjunctival epithelium, hypertrophic vascularized tissue, and degenerated connective tissue, while ultrastruc-. This content is licensed under a Creative Commons Attributions 4.0 International License. The proliferation and degeneration of collagen fibers play a huge role in the occurrence and development of pterygium, especially the expression of collagen I(6). Some studies have shown that high expression levels of TGF-β1 may play an important role in the development of pterygium[7], while other studies have confirmed that the transcription factor specificity protein 1 (Sp1) is a downstream target of TGF-β1 that plays a role in the synthesis and degradation of collagen I(8,9). The aim of the present study was to examine the expression profiles of Sp1 and collagen I in primary pterygium, and to explore the role and underlying mechanism of Sp1 in the regulation of pterygial development, thereby providing an experimental basis and new ideas for the further study of associated signal transduction pathways

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