Expression of special AT-rich sequence binding protein-1 in thyroid cancer and effect of special AT-rich sequence binding protein-1 on the invasion of thyroid cancer cells

Abstract

Objective To observe special AT-rich sequence binding protein-1 (SATB1) expression in thyroid cancer and the effect of SATB1 on invasion of thyroid cancer cells.Methods The expression of SATB1 in 85 cases of thyroid cancer was detected by using immunohistochemistry.The correlation between SATB1 and clinicopathological features was analvzed by Chi-Squared test.The effect of SATB1 on invasion of SW579 cells was analyzed by using invasion assay.Results The positive expression of SATB1 in thyroid cancer was 68.2％ (58/85).SATB1 was mainly expressed in nucleus of thyroid cancer cells.SATB1 expression was associated with lymph node metastasis and pathological stage (P ＜ 0.05),but not associated with sex,age,tumor sizes and number of tumor nodules (P ＞ 0.05).Invasion assay showed that the migrating number of empty vector transfected group and pEGFP1-SATB1 transfected group was respectively (220±10) and (416±15) (P＜0.05).Conclusion The up-regulation of SATB1 in thyroid cancer has a close relationship to the invasion of thyroid cancer cells. Key words: Thyroid neoplasm; Nuclear matrix protein; Special AT-rich sequence binding protein-1 ; Tumor metastasis