Expression of special AT rich sequence binding protein-1 in metastasis of prostatic carcinoma

Abstract

Objective To investigate the expression of special AT rich sequence binding protein (SATB-1) in prostatic carcinoma cell lines and prostatic carcinoma tissues,and its relationship with metastasis of prostatic carcinoma.Methods The immunohistochermistry streptavid-in-peroxidase (SP) method was used to detect the SATB-1 expression level in 30 cases of prostatic carcinoma with metastases and 30 cases of prostatic carcinoma without metastasis.The expression of SATB-1 mRNA and the invasive capability in 3 prostatic carcinoma cell lines (LNCaP,DU-145,PC-3) were measured by using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Transwell invasive assay.Results The score of staining intensity of SATB-1 in prostatic carcinoma samples with metastasis was higher than that in prostatic carcinoma samples without metastasis (P ＜ 0.05).There was statistically significant difference among the pathological Gleason scores (P ＜ 0.05).RT-PCR results revealed that the expression of SATB-1 was detectable in all cells.The mRNA expression ratio of SATB-1/β-actin in DU-145,PC-3 and LNCaP eells was 0.720 40 ±0.026 68,0.426 10 ±0.015 78,and 0.393 90 ±0.017 86 respectively.The number of in vitro invasive cells in DU-145,PC-3 and LNCaP cell lines was (98.60 ± 8.57),(68.50 ±7.15),and (42.70 ± 4.53) respectively.Conclusion SATB-1 was overexpressed in higher invasive prostatic carcinoma cells and prostatic carcinoma tissues with metastasis,and SATB-1 may play an important role in promoting metastasis of prostatic carcinoma. Key words: Prostatic carcinoma; Metastasis; Special AT rich sequence bindingprotein-1