EXPRESSION OF SOME IMMUNOLOGIC MARKERS AND THE RELATION BETWEEN TUMOUR CELL LINEAGE WITH HISTOPATHOLOGY IN NON - HODGKIN LYMPHOMA

Abstract

Background: Non - Hodgkin lymphoma is one of the two most common types of lymphoma, accounting for 85% and one of the most ten common cancers in the world as well as in Vietnam. The application of immunohistochemistry besides the histopathology for the tumours cell lineage diagnosis as well as the relation between the cell lineage with histopathology were very important for the prognosis and therapy orientation. Objectives: - To describe the gender, location and the malignant grade in patients with non – Hodgkin lymphôma; - To determine the expression of some immunologic markers, the relation between tumour cell lineage and histopathology in patients with non - Hodgkin lymphoma. Materials and method: This cross-sectional study was carried out on 60 patients with non- Hodgkin lymphoma diagnosed definitely by histopathology and classified as B, T cell by immunohistochemistry at Hue Central Hospital and Hue University Hospital. Results: The ratio of male/female for the non-Hodgkin lymphoma was 1.14/1, non - Hodgkin lymphoma appeared at lymph node was the most common (51.7%), at the extranodal site was relatively high 48.3%. The intermediate malignancy grade of non - Hodgkin lymphoma was the highest proportion accounting for 85%, then the low and the high ones 8.3% and 6.7% respectively. Immunophenotype by 5 markers including LCA, CD3, CD20, CD79a and CD45RO showed that: the B-cell non-Hodgkin lymphoma was predominant type (85%) and appeared more frequent as intermediate or low grade of malignancy, T - cell non – Hodgkin lymphoma was 13.3% and under the high orientation of malignant grade, unclassified type 1.7%; There were no relation between the tumours cell lineage and the gender or the original location of the tumours as well. Conclusion: The application of immunohistochemistry by 5 markers: LCA, CD3, CD20, CD79a and CD45RO was able to divide the tumour cell as the B or T cell of the non – Hodgkin lymphoma which was for for the prognosis and for the better treatment orientation as well. Key words: Lymphoma, non-Hodgkin lymphoma, grade, histopathology, immunohistochemistry, cell lineage