Abstract
IntroductionStomatin-like protein 2 (SLP-2), a member of the Stomatin superfamily, has been identified as an oncogenic-related protein and found to be up-regulated in multi-cancers. Nonetheless, the expression pattern and regulation of SLP-2 in human esophageal squamous cell carcinoma (ESCC) remain unexplored.MethodsImmunohistochemistry and immunofluorescence staining analysis were performed to show SLP-2 expression and location. RNAi method was used to inhibit specific protein expression. Transwell assay was done to investigate cells invasive capability. RT-PCR and Western blot analysis were used to detect mRNA and protein expression levels.ResultsImmunohistochemical analysis showed that up-regulation of SLP-2 was found in invasive front compared with cancer central tissue in ESCC. Inhibition of SLP-2 by SLP-2 siRNA can decrease ESCC cells invasive capability through MMP-2 dependent manner. Up-regulation of SLP-2 was effectively abrogated by the ERK1/2 inhibitors either PD98059 or U0126, but no effect was showed by the treatment of AKT inhibitors either LY294002 or MK-2206. So the regulation of SLP-2 was involved in activation of the MAPK/ERK pathway.ConclusionsWe found that PMA/EGF could induce the up-regulated expression of SLP-2 probably through activating ERK signalling. The current study suggests that SLP-2 may represent an important molecular hallmark that is clinically relevant to the invasion of ESCC.
Highlights
Stomatin-like protein 2 (SLP-2), a member of the Stomatin superfamily, has been identified as an oncogenicrelated protein and found to be up-regulated in multi-cancers
Bioinformatics analysis showed that SLP-2 is highly conserved in development, its conservative stomatin-like domain was observed in 51 other proteins with potentially diverse functions
The results showed that SLP-2 protein levels were higher in invasive front tissues than that in tumor central tissues (65%, 13/ 20)
Summary
Stomatin-like protein 2 (SLP-2), a member of the Stomatin superfamily, has been identified as an oncogenicrelated protein and found to be up-regulated in multi-cancers. The expression pattern and regulation of SLP-2 in human esophageal squamous cell carcinoma (ESCC) remain unexplored. By microarray analysis of various cancer tissues, we have led to the identification of multiple differentially expressed genes, which maybe serve as more important diagnostic or prognostic markers and even some new treatment targets for cancer. We screened a series of genes with diverse expression in esophageal squamous cell carcinoma (ESCC) tissues as compared with their normal counterparts by complementary DNA (cDNA) microarray. One gene named human stomatin-like protein 2 (SLP-2) was dramatically highly expressed [2,3,4]. Bioinformatics analysis showed that SLP-2 is highly conserved in development, its conservative stomatin-like domain was observed in 51 other proteins with potentially diverse functions
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