Abstract
Objective To investigate alterations in epigenetic regulation in B cells in subacute cutaneous lupus erythematosus (SCLE). Methods Peripheral blood mononuclear cells (PBMC) were isolated from the peripheral venous blood of SCLE patients and healthy donors with density gradient centrifugation. B cells were isolated with CD19 micro beads and protocols provided by the manufacturer. Total RNAs were isolated with RNA kits. The expressions of silence information regulator 1 (Sirt1) mRNA were investigated with quantitative real time polymerase chain reaction (PCR). Results The levels of Sirt1 mRNA were significantly increased in SCLE B cells relative to healthy controls (P< 0.01). Conclusions Histone modifications appear abnormal in B cells in SCLE. Key words: Lupus erythematosus, systemic/ME/GE; Sirtuins/ME/GE; B-lymphocytes
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
