Expression of Sex Hormone-Binding Globulin mRIMA in Uterine Cervical Cancers

Abstract

To explore the role of sex hormone-binding globulin (SHBG) in the intracellular steroidal actions in human uterine cervical cancers, the expression of SHBG mRNA as a manifestation of intracellular SHBG expression was investigated using the competitive reverse transcription-polymerase chain reaction-Southern blot analysis. The expression of SHBG mRNA was detected in all cervical endometria and cancers analyzed. The levels of SHBG mRNA in cervical cancers were significantly lower (p < 0.01) than in the normal cervical endometrium. In cervical cancers, the levels of SHBG mRNA in cervical adenocarcinomas were significantly higher (p < 0.01) than in keratinizing and small cell nonkeratinizing squamous cell carcinomas, and tended to be higher than in large cell nonkeratinizing squamous cell carcinomas. There was no difference in expression among the clinical stages of cervical cancers. These data suggest that human uterine cervical cancers, especially adenocarcinomas, might synthesize SHBG intracellularly, and might conserve the activity of SHBG-related steroidal mechanisms to some extent.