Abstract
B7H1 and B7H4 overexpression is associated with inhibition of the immune system in many solid tumors, and altogether with CD200 molecule plays an important role in tumor invasion by promoting malignant transformation. However, there is no report about impact of these molecules on laryngeal squamous cell carcinoma. The objective of the present study was to assess by means of flow cytometry the expression of B7H1, B7H4, CD200, and CD200R on CD83+ monocyte-derived dendritic cells (Mo-DC), pulsed with autologous tumor cell lysates (aTCL) in patients who suffer from G1, G2, or G3 laryngeal carcinoma (LC, n = 60) in comparison to healthy donors (HD, n = 15). It has been demonstrated that median value of the percentages of CD83+ B7H1+, CD83+ B7H4+, and CD83+ CD200+ cells were higher in LC patients than HD (p = 0.041, p ≤ 0.0001, and p = 0.02, respectively). Mean fluorescence intensity (MFI) of CD200, CD200R, B7H1, and B7H4 on the Mo-DC pulsed with aTCL of the patients was also higher than on the Mo-DC of HD (p ≤ 0.0001, p ≤ 0.0001, p = 0.002, and p ≤ 0.0001, respectively). The highest MFI levels of all molecules were noted in grade 3 LC. The aforementioned results prove that there is a relation between the presence of laryngeal cancer and the expression of B7H1, B7H4, CD200, and CD200R regulatory molecules on the CD83+ Mo-DC pulsed with autologous cancer cell lysates. Strong association of LC grade and the tested antigens expression suggests a critical role for these proteins in LC biology.
Highlights
Laryngeal cancer is one of the most commonly occurring malicious cancers of head and neck region, with squamous cell carcinoma as the predominant histologic type [1, 2]
The objective of the present study was to assess by means of flow cytometry the expression of B7H1, B7H4, CD200, and CD200R on CD83? monocyte-derived dendritic cells (Mo-DC), pulsed with autologous tumor cell lysates in patients who suffer from grade 1 (G1), grade 2 (G2), or grade 3 (G3) laryngeal carcinoma (LC, n = 60) in comparison to healthy donors (HD, n = 15)
The aforementioned results prove that there is a relation between the presence of laryngeal cancer and the expression of B7H1, B7H4, CD200, and CD200R regulatory molecules on the CD83? Mo-DC pulsed with autologous cancer cell lysates
Summary
Laryngeal cancer is one of the most commonly occurring malicious cancers of head and neck region, with squamous cell carcinoma as the predominant histologic type [1, 2]. In spite of their constant development, the classical therapeutic methods, such as surgical procedures or radiotherapy are not sufficiently effective in prolonging the survival time of cancer patients and improving the quality of their lives [3]. Overexpression of CD200 has been reported in a number of malignancies and on cancer stem cells [11] and this molecule may play roles in local tumor invasion as well as augments the metastatic capacity of squamous cell carcinoma [12]
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