Expression of scavenger receptor A in rat's liver tissue during acute obstructive cholangitis and its significance.

Zuojin Liu,Jianping Gong,Kang Yang,Akanand Singh

doi:10.1186/s40064-016-2222-5

Abstract

ObjectiveAcute obstructive cholangitis (AOC) is a severe disease that leads to sepsis, shock and multiple organ dysfunction syndrome. Liver is the largest and the major organ for the defense mechanisms during the sepsis. Our aim was to investigate the expression of scavenger receptor A (SRA) in rat’s liver tissue during AOC and its relation with the inflammatory mediators and hepatic injury caused by endotoxins.MethodsNinety Wistar rats were randomly divided into three equal groups. In group one, the choledochus were ligagted and Escherichia coli O111B4 injected into it to produce AOC model. Group two had choledochus ligated and group three had sham surgery. Six animals from each group were sacrificed at 0, 3, 6, 12 and 24 h for comparative analysis of endotoxins, tumor necrosis factor-alpha, interleukin-6 in plasma and expression of SRA protein and SRA mRNA in liver tissue. The P < 0.05 was considered significant. Ethical approval was obtained.ResultsThe plasma endotoxins, tumor necrosis factor-alpha and interleukin-6 levels in AOC rats increased progressively with time. The SRA protein and SRA mRNA expression decreased with time. The differences among groups were significant (P < 0.01). Liver histopathology showed gradually aggravated hepatic injury with neutrophil infiltration, degeneration and necrosis of hepatocytes.ConclusionIn AOC, the expression of SRA in liver gradually decreased with progressive increase in level of plasma endotoxins and hepatic injury suggesting its importance in the defense mechanism.

Highlights

Liver is vital organ which has many functions including immunological tolerance for foreign antigens
The changes of plasma endotoxin levels The plasma endotoxin concentrations in Acute obstructive cholangitis (AOC) group were higher at 3 h after surgery and increased progressively reaching peak at 24 h
The increase in level endotoxin in AOC group was significant compared to bile duct ligation group (BDL) and sham operation group (SO) group (P < 0.01)

Summary

Introduction

Liver is vital organ which has many functions including immunological tolerance for foreign antigens. Acute obstructive cholangitis (AOC) commonly leads to sepsis, septic shock and multiple organ dysfunction syndrome (MODS) (Takada et al 2013). Acute obstructive cholangitis (AOC) is a type of cholangitis with the symptom of right upper abdominal pain, jaundice, chills with fever. In severe cases of AOC, sign of central nervous system such as lethargy, Liver is the largest organ for defense in sepsis (Hilliard et al 2015). Kupffer cells play vital role in defense by clearing bacterium and endotoxin (Bilzer et al 2006; Hutchins et al 2013). They have important role in the pathogenesis of hepatic injury in sepsis and MODS

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