Abstract
Polyamines play an important role in plant response to abiotic stress. S-adenosyl-1-methionine decarboxylase (SAMDC) is one of the key regulatory enzymes in the biosynthesis of polyamines. In order to better understand the effect of regulation of polyamine biosynthesis on the shelf life improvement of litchi fruit, SAMDC cDNA isolated from Datura stramonium cloned in pBI121 was introduced into litchi genome by means of Agrobacterium tumefaciens through zygote disc transformation. Transgene and its expression are confirmed by Southern and Northern blot analyses, respectively. Transgenic plants expressing Datura SAMDC produced 1.7- to 2.4-fold higher levels of spermidine and spermine than wildtype plants under normal environmental condition, which indicated that the transgenic litchi presented an enhanced polyamines synthesis compared to wildtype plants. Our results demonstrated clearly that increasing polyamine biosynthesis in plants may be a means of creating improved fruit shelf life germplasm.
Highlights
Litchi (Litchi chinensis Sonn.) is native to Southern China, which is adapted to the warm subtropics, croppingHow to cite this paper: Das, D.K., Prabhakar, M., Kumari, D. and Kumari, N. (2016) Expression of S-adenosyl-1-methionine decarboxylase (SAMDC) Gene for Enhancing the Shelf Life for Improvement of Fruit Quality Using Biotechnological Approaches into Litchi (Litchi chinensis Sonn.) Cultivars
The combination of ascorbic acid (AA) and citric acid (CA) increased the percentage of somatic embryogenesis and the development of the somatic embryos
Primary somatic embryos were generated from zygotic embryos when cultured on MS1 medium, they were subcultured on either NN basal or NN medium supplemented with IBA and within 4 - 6 weeks embryogenic callus was formed (Figure 3(a)), which differentiated into many globular to cotyledonary stage secondary embryos
Summary
Litchi (Litchi chinensis Sonn.) is native to Southern China, which is adapted to the warm subtropics, croppingHow to cite this paper: Das, D.K., Prabhakar, M., Kumari, D. and Kumari, N. (2016) Expression of SAMDC Gene for Enhancing the Shelf Life for Improvement of Fruit Quality Using Biotechnological Approaches into Litchi (Litchi chinensis Sonn.) Cultivars. (2016) Expression of SAMDC Gene for Enhancing the Shelf Life for Improvement of Fruit Quality Using Biotechnological Approaches into Litchi (Litchi chinensis Sonn.) Cultivars. It has a short shelf-life under normal ambient conditions due to skin color loss (browning) and deterioration during storage and transportation [2]. The fruit loses its commercial value after harvest due to pericarp browning, quality deterioration and decay [3]. Browning of litchi pericarp is still considered to be a major problem affecting its market value. Browning of litchi pericarp was thought to be due to degradation of anthocyanidin by polyphenol oxidase (PPO) and peroxidase (POD) [4] [5] and was primarily the result of PPO activity degrading the anthocyanins and producing brown-coloured by-products [6]
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