Abstract
Objective To investigate the Rho dissociation inhibitor 2 (RhoGDI2) gene expression in colon cancer tissues and its correlation with clinicopathological features.Methods Tissue microarray and immunohistochemistry were used to detect the expression of RhoGDI2 in the primary lesions and adjacent colon tissues of 75 cases of colon cancer,and its correlation with clinicopathological features of colon cancer was analyzed.Western blotting and real-time polymerase chain reaction (Real-time PCR) were used to examine the RhoGDI2 expression in the primary lesions and adjacent colon tissues of 18 cases of colon adenocarcinoma.Results The positive RhoGDI2 expression rate in primary lesions and adjacent normal tissues of colon cancer was 73.33％ and 33.33％ respectively (P ＜ 0.05).The positive expression rate of RhoGDI2 was correlated with TNM staging significantly (P ＜ 0.05) and primary tumor T lymph node N stage related to the scope (P ＜ 0.05),but not with distant metastasis M and lymph node N stage (P＞0.05).The gray values of RhoGDI2 in 18 cases of colon adenocarcinoma tissues were 4.8 times higher than normal tissues (P ＜ 0.05).Real-time PCR results revealed that the relative expression of RhoGDI2 in the primary lesions was higher than in control group (P ＜ 0.05).Conclusion Up-regulation of RhoGDI2 expression in colon cancer is significantly correlated with TNM staging.RhoGDI2 may be one of the colon cancer prognosis factors and the potential therapeutic targets. Key words: Colon carcinoma; Tissue microarray; Rho dissociation inhibitor 2
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