Expression of retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) in the human term placenta

Abstract

Event Abstract Back to Event Expression of retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) in the human term placenta Agnieszka Jabłońska1, Patrycja Suski1, Mirosława Studzińska1, Jarosław Kalinka2 and Edyta Paradowska1* 1 Institute of Medical Biology Polish Academy of Sciences, Laboratory of Molecular Virology and Biological Chemistry, Poland 2 Medical University of Lodz, Department of Perinatology, First Chair of Gynecology and Obstetrics, Poland Innate recognition of viruses is mediated by germline-encoded pattern recognition receptors (PRRs) that initiate signaling events, resulting in the production of type I interferon and proinflammatory cytokines. Double-stranded RNA and 5’triphosphate group of ssRNA generated during viral replication are detected by sensory molecules, named RIG-I-like receptors (RLRs). Three members of the RLRs family have been characterized so far, including RIG-I (retinoic acid-inducible gene-I), MDA5 (melanoma differentation-associated gene 5) and LGP2 (laboratory of genetics and physiology 2). RIG-I participates in the recognition of Paramyxoviruses, Rhabdoviruses, Flaviviruses and Orthomyxoviruses, whereas MDA-5 is essential for the recognition of Picornaviruses. In this study, we investigated the RLRs gene expression in fetal and maternal parts of the human placentas during viral infection. The explants of third-trimester chorionic villi and deciduas were exposed to infection with dsDNA viruses (human cytomegalovirus, HCMV; herpes simplex virus type 1, HSV1) or ssRNA virus (vesicular stomatitis virus, VSV). RIG-I, MDA5 and LGP2 mRNA levels were assessed by real-time PCR. Our results demonstrated that all RLRs were expressed in term human placentas with the highest level of LGP2. RIG-I and MDA5 (in fetal part) have been identified as the main mediators of VSV recognition, whereas RLRs were not involved in the identification of dsDNA viruses. The expression of LGP2 in HCMV-infected explants was decreased compared to mock-infected. The results clearly indicated that RLRs play essential role in RNA viruses’ recognition in term placenta. Acknowledgements Project financed by the European Regional Development Found under the Operational Programme Innovative Economy, Grant No. POIG.01.01.02-10-107/09. Keywords: human placenta, Virus infection, RLRs, RIG-I-like receptors, LGP2 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Jabłońska A, Suski P, Studzińska M, Kalinka J and Paradowska E (2013). Expression of retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) in the human term placenta. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00293 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Edyta Paradowska, Institute of Medical Biology Polish Academy of Sciences, Laboratory of Molecular Virology and Biological Chemistry, Lodz, 93-232, Poland, eparadowska@cbm.pan.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Agnieszka Jabłońska Patrycja Suski Mirosława Studzińska Jarosław Kalinka Edyta Paradowska Google Agnieszka Jabłońska Patrycja Suski Mirosława Studzińska Jarosław Kalinka Edyta Paradowska Google Scholar Agnieszka Jabłońska Patrycja Suski Mirosława Studzińska Jarosław Kalinka Edyta Paradowska PubMed Agnieszka Jabłońska Patrycja Suski Mirosława Studzińska Jarosław Kalinka Edyta Paradowska Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.