Abstract
The fungus Ramularia collo-cygni is increasingly important as the causal agent of Ramularia leaf spot (RLS), a novel leaf spot disease of barley. The work aimed to identify gene resources suitable for developing new breeding lines of winter barley with improved resistance. During the first experimental period (2001–2005), RLS incidence was monitored in 711 cultivars and advanced breeding lines. Differences were detected in the intensity of symptomatic expression, but no material showed high resistance. During the second experimental period (2006–2009), response to natural RLS infection was evaluated in 19 winter barley cultivars (12 six-row and 7 two-row) registered in the Czech Republic. Highest susceptibility was detected in 6-row cvs. Luran, Laverda and Wendy while cvs Breunskylie (2-row), Merlot and Highlight (both 6-row) showed relatively lower disease incidence. High resistance was not detected. On average, 2-row cultivars showed lower intensity than 6-row cultivars and significant variation was observed among years.
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