Abstract
Nonrandom distribution of meiotic recombination events along the chromosomes shapes the diversity of potential genetic combinations among the offspring. To redistribute the chromosomal regions involved in recombination events, it was proposed to use meiosis-specific genes of Spo11 proteins (generating double-strand DNA breaks) from phylogenetically different organisms. For these purposes, transgenic tomato plants expressing native SPO11 genes from yeast (Saccharomyces cerevisae) or Arabidopsis thaliana under the control of constitutive 35S CaMV promoter were constructed. Genetic analysis showed that expression of both target SPO11 genes partly disturbed the monogenic inheritance of marker Wv:wv alleles in chromosome 2 and suppressed the crossing over in the region between the wv and d genes. A stable negative correlation between the target gene expression levels and the decrease in the frequency of crossing over in the analyzed chromosomal region was found. The possible genetic mechanisms underlying the redistribution of crossovers along chromosome 2 resulting from the target SPO11 gene expression are discussed.
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