Abstract
cDNA clones encoding human plasminogen (PLGN) and aglyco (Asn289 → Gln,Thr346 → Ala) plasminogen (APLGN) were expressed under the control of the Rous sarcomavirus long terminal repeat (RSVLTR) in a HeLa cell transient expression system. Harvest medium was analysed for the presence of recombinant PLGN using three streptokinase (SK)-dependent methods; (1) a direct S2251 amidolytic assay; (2) a plasminogen activator assay and (3) fibrin zymography after electrophoresis. Our results are consistent with the expression of authentic PLGN (both type I and type 11) and APLGN.
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