Expression of reactive oxygen species in prostatic stromal cells in hypoxic environment and its effect on the cell growth

Abstract

Objective To explore expression of reactive oxygen species in human prostatic stromal cells(PSC)in hypoxic environment and its effect on the cell growth. Methods Human prostatic stromal cells from patients with benign prostatic hyperplasia and normal subjects were isolated.The expression of ROS in PSC was detected by flow cytometry assay.The mRNA expression level of hypoxia-inducible factor-1α(HIF-1α), androgen receptors(AR), vascular endothelial growth factor(VEGF)and interleukin-8(IL-8)in PSC was detected with the real-time quantitative RT-PCR method.Under the intervention with versus without Edaravone, we measured the change of cells growth and ROS, and mRNA expression level of HIF-1α, AR, VEGF and IL-8 under hypoxic conditions. Results The expression of ROS in tissues and cells was significantly increased under hypoxic condition(P<0.01).3% O2 promoted the proliferation of prostatic stromal cells.The mRNA expressions of HIF-1α, AR, VEGF and IL-8 were upregulated under 3% O2(all P<0.05). After Edaravone intervention the ROS was significantly decreased, and mRNA expression levels of HIF-1α and VEGF were down regulated(all P<0.05), and cell proliferation declined(P<0.01). Conclusions Hypoxia stimulates the generation of ROS, and the ROS may play a key role in development of benign prostatic hyperplasia. Key words: Reactive oxygen species; Cell hypoxia; Prostatic hyperplasia; Stromal cells