Abstract
Objective To explore the expression of wild-type p53-induced phosphatase 1 (Wip1)in astrocytomas and its clinical significance. Methods In 32 cases of high-grade astrocytomas, 35 cases of low-grade astrocytomas and 10 cases of normal brain tissues the expression of Wip1 mRNA and protein was detected by using immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR)and Western blotting. The expression of p53 was assayed in Wip1 positive tissues by using immunohistochemistry and Western blotting. The relationship between the Wip1 expression and clinicopathological features was analyzed. Results ( 1 ) Immunohistochemistry : The positve expression rate of Wip1 in highgrade astrocytoma, low-grade astrocytoma and normal brain tissues was 24/32 (75.0%), 11/35(31.4%) and 1/10 (10.0%) respectively. There was significant difference among three tissues (P＜0. 05 ). (2) RT-PCR: The expression level of Wip1 mRNA in high-grade astrocytoma, low-grade astrocytoma and normal brain tissues was 0. 35 ±0. 07, 0. 07±0. 03 and 0. 05 ±0. 02 respectively. The expression level of Wip1 mRNA in high-grade astrocytoma was higher than that in low-grade astrocytoma and normal brain tissues (P ＜0. 05). (3) Western blotting: The relative expression of Wipl protein in high-grade astrocytoma, low-grade astrocytoma and normal brain tissues was 1.67 ±0. 12, 1.05 ±0.25 and 0.41 ±0. 16 respectively, p53 immunohistochemical staining revealed that only 13 were positive for p53 in 36Wip1 positive tissues. The high expression rate of Wip1 protein was negatively correlated with the expression of p53 ( r = - 0. 29). The relative expression of p53 protein in high-grade astrocytoma, low-grade astrocytoma and normal brain tissues was 0. 77 ±0. 09, 0. 63 ±0. 13 and 0. 21 ±0. 11 respectively. They was no statistically significant difference between high-grade astrocytoma and low-grade astrocytoma (P ＞0. 05), but the relative expression of p53 protein in high-grade astrocytoma, low-grade astrocytoma was higher than in normal brain tissues ( P ＜ 0. 05 ). The expression of Wip1 was greatly correlated with its pathologic classification ( P ＜ 0. 05 ), but it had nothing to do with tumor size and age ( P ＞ 0. 05 ). Conclusion The high expression of Wipl mRNA and protein in astrocytoma is related to its pathologic classification and negatively correlated with the expression of p53. Wip1 may play an important role in the tumor development. Key words: Astrocytoma; p53; Treatment
Published Version
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