Abstract
We previously reported an anatomical connection from cardiovascular (CV)‐related brain nuclei to the bone marrow (BM) and an alteration of mesenchymal stem cell (MSC) colony‐forming ability during Ang II‐induced neurogenic hypertension. This study was conducted to determine if renin‐angiotensin system (RAS) components exist in BM‐related neurons, which will lay a foundation for study of the role of brain RAS in MSC regulation during hypertension. A retrograde pseudorabies virus (PRV) with a green fluorescent protein (GFP) tag was injected into the femoral bone marrow of mice (n=3). Six days post‐inoculation, mice were perfused with 4% paraformaldehyde and brains were harvested for immunostaining for GFP, angiotensin II type 1 receptor (AT1R), and (pro)renin receptor (PRR). PRR and AT1R were localized in BM‐related neurons in several CV regulatory brain regions, including the nucleus of the solitary tract, the rostral ventrolateral medulla, and most notably in the paraventricular nucleus (PVN) of the hypothalamus. The data suggests that key receptors of brain RAS are present in the BM‐related neurons and might play a role in the regulation of MSCs during the development of neurogenic hypertension.
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