Abstract
Objective To investigate the regulatory role of phospholipid scramblase 1 (PLSCR1) during the growth and metastasis of hepatic carcinoma.Methods The PLSCR1 interference cell lines were constructed.The significance of PLSCR1 during cell proliferation and invasion was measured by methyl thiazol tetrazolium (MTT) assay and Invasion assay.The expression of PLSCR1 was detected by real-time fluorescent quantitative polymerase chain reaction(FQ-PCR) in 20 patients with hepatic carcinoma.Results The PLSCR1-small interfering RNA (siRNA)-HepG2 cell lines were successfully constructed.It was found the PLSCR1 interference could suppress the growth and invasion,respectively.the inhibition rate is separately (28.71 Ā± 1.27(ļ¼ and (27.16 Ā± 10.40) ļ¼ .The results of FQ-PCR indicated PLSCR1 was overexpressed in tumor tissue (ā³/ā³ Ct:-2.57 Ā± 1.34),and it was related with the tumor differentiation and clinical stages (P ļ¼ 0.05).Conclusion PLSCR1 is over-expressed in hepatic carcinoma,and has close correlation with tumor growth and progress. Key words: Phospholipid scramblase 1 ; Carcinoma, hepatocellular
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