Abstract
Objective To investigate the role of platelet-derived growth factor B (PDGF-B) expression levels and the methylation levels of its gene promoter on proliferation of human gliomas.Methods The expressions of PDGF-B,p-Smad2 and Ki-67 were determined by immunohistochemistry in 54 human glioma specimens.And the methylation levels of PDGF-B gene promoter were determined using pyrosequencing.Results (1) The expression levels of PDGF-B increased with the pathologic grades elevated (P ＜0.05).(2) There was a positive and significant correlation between the expression levels of PDGF-Band Ki-67 as well as p-Smad2 (r =0.839,P ＜0.001) (r =0.802,P ＜0.001),respectively.And an inverse and significant correlation was found between the expression levels of PDGF-B and its gene promoter methylation (r =-0.817,P ＜0.001).(3) The methylation levels of PDGF-B gene promoter were significantly higher in low grade gliomas (WHO Ⅰ Ⅱ) than those in high grade gliomas (WHO Ⅲ Ⅳ) (P =0.001).Conclusions The expression levels of PDGF-B and its gene promoter methylation play an important role in predicting the malignancy and proliferation of human gliomas. Key words: Glioma; Gene Methylation; Pyrosequencing; Platelet-derived growth factov B
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