Expression of PCNA, MMP-9, p53, Bax and Bcl-2 in canine transmissible venereal tumors

Emin Karakurt,Özhan Karataş,Serpil Dağ,Hilmi Nuhoğlu,Murat Can Demi̇r,Ayfer Yildiz,Mushap Kuru,Cihan Kaçar,Enver Beytut

doi:10.30704/http-www-jivs-net.700692

Abstract

In this study, we aimed to evaluate the proliferative, metastatic and apoptotic capacities of TVT cases, which are in various phases of development, by using immunohistochemical markers. The material of this study consisted of twelve female and six male dogs diagnosed with TVT brought to our department between 2007 and 2020 years. Diff-quick staining was applied to the smear taken from tumoral masses for cytological examinations. Tumoral tissues from dogs were fixed in a 10% neutral buffered formaldehyde solution. After routine tissue procedures Hematoxylin &amp; Eosin stain was applied to the sections. Tissue sections were investigated under a light microscope and photographed. Immunohistochemical staining was performed on the tissues using the avidin-biotin immune peroxidase complex method. As a result of macroscopic, cytological and histopathological examinations, TVT positive cases were divided into three according to their developmental stages. While the expression of PCNA, MMP-9, mutant p53 and Bcl-2 increased significantly in progressive cases compared to regressive and stable cases, Bax expression increased significantly in regressive cases compared to progressive and stable cases. In conclusion, we thought that the mentioned markers are very useful for understanding the prognosis of TVT, the tumor aggressiveness and the survival of the malignant cells.

Highlights

Transmissible venereal tumor (TVT) knowns as mostly found in sexually active, free-circulating, infectious sarcoma, venereal granuloma, young dogs in many regions of the world (Akkoc et. transmissible lymphosarcoma or Sticker tumor, is al., 2017)
We aimed to evaluate the Ratio: 1/100), (MMP-9: Santa Cruz, sc-393859, proliferative, metastatic and apoptotic capacities Dilution Ratio: 1/100), (p53: Novus Bio, SPM590, of TVT cases, which are in various phases of Dilution Ratio: 1/200), (Bax: Bioss, bs-0127R, development, by using immunohistochemical Dilution Ratio: 1/200) and
We evaluated a highly reliable marker Proliferating cell nuclear antigen (PCNA) expression in order to compare the stages of development of TVT cases in terms of cell proliferation (Chu et al, 2001)

Summary

Introduction

Transmissible venereal tumor (TVT) knowns as mostly found in sexually active, free-circulating, infectious sarcoma, venereal granuloma, young dogs in many regions of the world (Akkoc et. transmissible lymphosarcoma or Sticker tumor, is al., 2017). Transmissible venereal tumor (TVT) knowns as mostly found in sexually active, free-circulating, infectious sarcoma, venereal granuloma, young dogs in many regions of the world Transmissible lymphosarcoma or Sticker tumor, is al., 2017). TVT, which has a higher incidence in the most common genital tumor in dogs (Özyiğit tropical and subtropical regions, mostly affects et al, 2014; Paramjeet et al, 2019). It is known to affect other canids. We divided these 18 animals into al., 2011a). It has been reported that this tumor is 3 phases (progression, stable and regression) seen with the same frequency in both female and including 6 of them

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Conclusion