Expression of P53, HER2 and Ki67 proteins in rats subjected to bladder augmentation with stomach, colon and ileum.

Edinaldo Gonçalves De Miranda,Jaques Waisberg,José Figueredo Silva,Diógenes Miranda Santana,Thiago Pereira Diniz,Antônio Igor Paraiba Lopes,Celina Teresa Castelo Branco Couto De Miranda Miranda,João Emílio Pinheiro Lemos Filho

doi:10.1590/s0102-865020160010000007

Edinaldo Gonçalves De Miranda, Jaques Waisberg + Show 6 more

Open Access

https://doi.org/10.1590/s0102-865020160010000007

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Abstract

To study the expression of HER2, p53 and Ki67 proteins in cystoplasties. Sixty rats were distributed randomly into three groups of 20 animals. Bladder augmentation was held to increase with ileum (Group I), colon (Group II) and stomach (Group III). Tissue samples of neobladder was collected from each rat to its own control. The animals were sacrificed after 12 weeks. The neobladder was withdrawn for immunohistochemistry analysis of p53, HER2 and Ki67 expression. Wilcoxon and Mann-Whitney tests were used for statistical study. There were no significant changes in the expression of p53 and HER2 proteins. It was observed significant increase (p<0.0001) in Ki67 expression in all groups, when compared with their respective controls. When the study groups were compared with each other, there was increase of cell proliferation in the largest gastrocystoplasties in respect of ileocystoplasties (p=0.004) and colocystoplasties (p=0.003). We observed significant increase of cell proliferation characterized by Ki67 protein in the digestive tract of the ileocystoplasties, the colocystoplasties and the gastrocystoplasties and this increase was significantly greater in gastrocystoplasties.

Highlights

Several diseases affect the lower urinary tract and require the use of digestive tract into the urinary derivation to partial or total replacement of the bladder to preserve renal function and reestablish the excretory urinary function[1]
The presence of changes in Ki67 protein expression was detected in tissue samples of ileum of control grups like in the ileociystoplasty group, (Ic), in the of colocystoplasties (Group IIc) and gastric segment of gastrocystoplasties (Group IIIi) (Figure 3), but without significance when compared with each other: Ic x IIc (p = 0.3425), Ic x IIIc (p = 0.2538) and IIc x IIIc (p = 0.1337)
The carcinogenesis can be triggered by prolonged contact between urine and the mucosa of the intestinal segment used to urinary tract reconstruction[15]

Summary

Introduction

Several diseases affect the lower urinary tract and require the use of digestive tract into the urinary derivation to partial or total replacement of the bladder to preserve renal function and reestablish the excretory urinary function[1]. The ileum was and continues to be the most used[3], but it is used colon, stomach, mucolized or demucolized, ureter, myoperitoneal patchwork, placenta, dura omentum, bovine pericardium, bladder auto enlargement techniques, such as detrusorectomies, botulinum toxin application and genetic engineering techniques with homologous and heterologous tissues , as well as bladder transplant and synthetic bladder were described[4,5,6] Each of these techniques has advantages and disadvantages and its use should be individualized, because complications may occur such as metabolic disorders, mucoid discharge, visceral pain distension, spontaneous perforation, calculations and urinary infections, hemorrhagic cystitis, complications with ventriculoperitoneal shunt, vesico-ureteral reflux, urinary loss, intestinal disorders, reproductive alterations in female and tumors development[7]

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