Expression of p110, phosphorylation-protein kinase B and protein kinase B in hemangioma vascular endothelial cell in vitro

Abstract

Objective To study the expression of phosphatidy linositol-3-kinase(PI3K) p110 catalytic subunit and phosphorylation-protein kinase B(p-Akt), protein kinase B(Akt) in cultured vascular endothelial cells, then the level of PI3K p110, p-Akt and Akt expression and the correlation with hemangioma vascular endothelial cell cycle and apoptosis were analyzed. Methods The hemangioma vascular endothelial cells which came from the sterile hemangioma were cultured by using tissue culture method and then synchronized cultured endothelial cells, PI3K p110, p-Akt and Akt of them were detected by way of Western blot, meanwhile cell cycle and cell apoptosis were examined by using flow cytometry technique, then the level of PI3K p110, p-Akt and Akt expression and the correlation with hemangioma vascular endothelial cell cycle and apoptosis were analyzed. Results The percentage of vascular endothelial cell was (94.00±3.00)% in the G0/G1 phase and the apoptosis rate was (11.24±2.34)% when p110 and p-Akt protein expression level were lower (0.19±0.01, 0.09±0.02), and they were (61.00±6.00)% and (0.51±0.03)% when p110 and p-Akt protein expression level were higher (0.37±0.04, 0.22±0.01). The percentage of vascular endothelial cell in the G0/G1 phase and the apoptosis rate were different in 2 groups(t=-8.42, -35.48, all P<0.01). But Akt protein expression level was not changed(0.72±0.00). Conclusions p110, p-Akt and Akt protein were expressed in the cultured hemangioma vascular endothelial cells in vitro.PI3K p110, p-Akt may be involved in cultured hemangioma vascular endothelial cells apoptosis in vitro. Key words: Hemangioma; Vascular endothelial cell; Cell culture; p110; Phosphorylation-protein kinase B; Protein kinase B; Cell apoptosis