Expression of Notchl and Jaggedl Proteins in Acute Myeloid Leukemia Cells

Abstract

Cell fate of hematopoietic progenitors is regulated by interaction between Notch proteins on progenitors and Notch ligands such as Jaggedl on stromal cells. Since acute myeloid leukemia (AML) originates from dysregulated hematopoietic progenitors, some abnormalities in the Notch-Jagged system may exist in AML cells. As the first step to clarify this, we examined the expression of Notchl and Jaggedl proteins in eight AML cell lines and 15 fresh AML samples by immunoblotting. In the Notchl protein, two bands, a 300 kDa band and a 120 kDa band, which appeared to be a full-length protein and a transmembrane fragment, respectively, were recognized in five AML cell lines and six fresh samples. In addition, three of the five cell lines showed a 110 kDa fragment, which appeared to be from an intracellular domain, namely an active form. One cell line showed aberrant sized fragments, which suggested a structural abnormality. Jaggedl protein was recognized in six cell lines and six fresh samples. In four cell lines and four fresh samples, both Notchl and Jaggedl proteins were observed. In these cells, Notchl and Jaggedl proteins may interact among themselves. We showed that Notchl and Jaggedl proteins are widely expressed in AML cells. We hypothesize that some abnormalities in the Notch-Jagged system which cause the excessive self-renewal and the block of differentiation, may be involved in the abnormal proliferation of AML cells.