Abstract
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) regulates intestinal immunological homeostasis. However, precise expression patterns of CEACAM1 isoforms remain poorly understood in the intestinal epithelia. Focusing on the small intestinal epithelium of BALB/c mice, we identified three novel splice variants encoding CEACAM1 a-2, -2C1, and -4C1 by RT-PCR. CEACAM1 a-2, -2C1, and -4C1 demonstrated secretory properties by transfection experiments in vitro. Among them, CEACAM1 a-4C1 was the major secreted isoform in vivo due to the soluble/secreted CEACAM1 a with a frameshift sequence in the C-terminus, specific for CEACAM1 a-2C1 and -4C1. CEACAM1 a-4C1 was capable of binding murine hepatitis virus (MHV) and was detected at approximately 120 kDa in the small intestinal secretions. Neutralizing effects of the soluble CEACAM1 a on MHV infectivity in vitro were demonstrated by using recombinant CEACAM1 a-4C1. Our data suggest an intrinsic mechanism operated by free CEACAM1 for surveillance of pathogens and maintenance of homeostasis in the intestine.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
