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Abstract
Conflicting reports of the distribution of sugarcane neutral invertase (SNI) activity in sugarcane, and the complete lack of genetic information have been the impetus for this investigation. Using a hetrologous sequence from Lolium temulentum a clone, which contained a 1716 bp open reading frame, was isolated from a mature culm sugarcane cDNA library. The SNI amino acid sequence had at least 53% homologous with sequences from Daucus carota and L. temulentum neutral invertases. The predicted molecular weight of the polypeptide is 64 300 Da. Southern blot analysis showed that SNI represents a low copy number gene in sugarcane. Both the SNI transcript and protein were present in all analysed tissues of the sugarcane plants and the data are consistent with transcriptional control of SNI activity. In culm tissues where sucrose content was low and hexose contents were high, SNI transcript and protein levels were higher than in tissues dedicated to sucrose storage.
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