Abstract
A cloned cell line derived from mouse bone marrow and transformed by Abelson virus is shown to synthesize two different heavy chains, mu and gamma 2B, in vitro. This characteristic is stable because it persists upon subcloning. Although most of the immunoglobulin-synthesizing cells produce either mu or gamma 2B heavy chains, a few cells contain both heavy chains, suggesting immunoglobulin class switching. Karyotypes show a complement of 41 chromosomes. Two copies of chromosome 12, to which immunoglobulin heavy chain structural genes have been assigned, were found. No light chain was found in either the mu- or the gamma 2B-producing cells. However, fusion of the cell line with a myeloma that synthesizes neither heavy nor light chains caused expression of kappa light chain in the hybridoma synthesizing mu chain. No light chain could be detected in the hybridomas synthesizing gamma 2B heavy chain.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Proceedings of the National Academy of Sciences of the United States of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.