Abstract
BackgroundAltered expression of miRNA expression contributes to human carcinogenesis. This study was designed to detect aberrant miRNA expressions as a potential biomarker for early detection and prognosis prediction of non-small cell lung cancer (NSCLC).MethodsmiRNA array was used to profile differentially expressed miRNAs and Taqman-based quantitative RT-PCR assays were used to analyze levels of miR-29c, miR-93, and miR-429 expression in NSCLC tissue samples, corresponding normal tissue samples, and serum samples from 70 NSCLC patients as well as in serum samples from 48 healthy controls.ResultsLevels of miR-29c and miR-93 expression were upregulated in NSCLC tissues, while serum levels of miR-29c were also upregulated, but levels of serum miR-429 were decreased in NSCLC. Moreover, the levels of miR-429 expression in NSCLC tissues were associated with those in serum samples. Receiver operating characteristic (ROC) curve analysis showed that at the optimal cut-off point, the areas under the ROC curve for serum levels of miR-29c and miR-429 were 0.723 and 0.727, respectively, levels which are higher than that of carcinoma embryonic antigen (0.534) in diagnosis of stage I NSCLC. In addition, serum levels of miR-429 were associated with poor overall survival of NSCLC patients. Both univariate and multivariate analyses showed that serum miR-429 level was an independent prognostic predictor for NSCLC.ConclusionsThe results of the current study suggest that detection of serum miR-29c and miR-429 expression should be further evaluated as a novel, non-invasive biomarker for early stage NSCLC.
Highlights
MicroRNAs are a class of small non-coding RNAs that can function as endogenous RNA interference to regulate expression of the targeted genes [1,2,3]
The results of the current study suggest that detection of serum miR-29c and miR-429 expression should be further evaluated as a novel, non-invasive biomarker for early stage non-small cell lung cancer (NSCLC)
A total of 1048 human miRNAs have been identified and reported in the RNA. Database, and this number continues to increase. Some of these miRNAs have been reported to be useful as potential biomarkers for diagnosis, prognosis, and personalized therapy of human cancers [1,2,3,4,5,6], because altered expression of these miRNAs contributes to human carcinogenesis
Summary
MicroRNAs (miRNAs) are a class of small non-coding RNAs that can function as endogenous RNA interference to regulate expression of the targeted genes [1,2,3]. A total of 1048 human miRNAs have been identified and reported in the RNA database, and this number continues to increase. Some of these miRNAs have been reported to be useful as potential biomarkers for diagnosis, prognosis, and personalized therapy of human cancers [1,2,3,4,5,6], because altered expression of these miRNAs contributes to human carcinogenesis. Aberrant miRNA expression has been reported in lung cancer. This study was designed to detect aberrant miRNA expressions as a potential biomarker for early detection and prognosis prediction of non-small cell lung cancer (NSCLC)
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