Expression of miR-23a and miR-135 and tumor markers in gastric cancer patients and the significance in diagnosis.

Abstract

Correlation between the expression of miR-23a and miR-135 and tumor markers in gastric cancer patients and their significance in diagnosis was investigated. A total of 78 patients with gastric cancer admitted to Dongying People's Hospital from July 2015 to June 2017 were enrolled, and 80 healthy patients were selected as the control group during the same period. The expression levels of miR-23a and miR-135 in the serum of the two groups were detected by RT-qPCR, and the expression levels of tumor markers CEA and carbohydrate antigen 199 (CA199) were detected by ELISA. The receiver operating characteristic curve and area under the curve (AUC) were used to evaluate the diagnostic efficacy of miR-23a and miR-135 as diagnostic indicators. There was no significant difference between the observation and the control group (P>0.05). The expression levels of miR-23a and miR-135 in the observation group were significantly higher than those in the control group (P<0.05). The expression levels of CEA and CA199 in serum of patients in the observation group were significantly higher than those in the control group (P<0.05). Pearson's correlation test showed that the expression levels of miR-23a and miR-135 were positively correlated with CEA and CA199 (P<0.05). The specificity, sensitivity and AUC of miR-23a in the diagnosis of gastric cancer were 67.95, 87.50 and 0.805%, respectively. The specificity, sensitivity and AUC of miR-135 in the diagnosis of gastric cancer were 73.08, 82.50 and 0.824%, respectively. Both could be used in the diagnosis of gastric cancer. In conclusion, miR-23a and miR-135 are highly expressed in gastric cancer patients and positively correlated with tumor markers, which can be used in diagnosis for gastric cancer.