Expression of miR-155 and its clinical significance in tissues of colonic cancer

Abstract

Objective To investigate the expression of miR-155 in the tissues of colonic cancer,and the relationship between miR-155 and the clinical pathological parameters of colonic cancer.Methods Fifty-seven samples of primary colonic cancer tissues and adjacent normal tissues were collected from Xiangyang Center Hospital from March to September,2011.The total RNAs were extracted from the colonic cancer tissues and normal tissues to conduct the reverse transcription reaction.The expressions of miR-155 in the colonic cancer tissues and normal tissues were detected using real time quantitative reverse transcription-polymerase chain reaction,and the relation-ship between the expression of miR-155 and the clinical pathological parameters of colonic cancer was analysed.Paire samples were analyzed using non-parametric Wilcoxon test,and independent samples were analyzed using the u test.Results The relative expression of miR-155 in the colonic cancer tissues was 0.421 (range,0.016-1.241),which was significantly higher than 0.128 (range,0.000-0.337) in the normal tissues (u =3.783,P ＜0.05).The increase of miR-155 expression was correlated with TNM staging,tumor invasion,lymphatic metastasis and tumor cell differentiation (u =2.364,2.152,2.338,2.214,P ＜ 0.05).Conclusions The expression of miR-155 is up-regulated in colonic cancer tissues.MiR-155 may play an important role in the invasion and metastasis of human colonic cancer. Key words: Colonic neoplasms ; Micro RNA ; miR-155