Abstract
Simple SummaryProstate cancer is a major cause of health loss and death worldwide, and better tools to assess risk levels in individual patients are needed. MicroRNAs (miRNAs) are small molecules with critical regulatory roles in cell functions and are also involved in prostate cancer development. The aim for this study was to investigate the role of miR-24-1-5p regarding prognosis in men diagnosed with prostate cancer and treated with radical prostatectomy. We collected prostate cancer tissue from 142 men already enrolled in a population-based cohort study who underwent prostatectomy. We examined the tissue expression of miR-24-1-5p in prostate cancer using in situ hybridization (ISH) and semi-quantitative scoring. We found that a high miR-24-1-5p expression was associated with a doubled risk of recurrence of prostate cancer.The role of miR-24-1-5p and its prognostic implications associated with prostate cancer are mainly unknown. In a population-based cohort, the Prostate Cancer Study throughout life (PROCA-life), all men had a general health examination at study entry and were followed between 1994 and 2016. Patients with available tissue samples after a prostatectomy with curative intent were identified (n = 189). The tissue expression of miR-24-1-5p in prostate cancer was examined by in situ hybridization (ISH) in tissue microarray (TMA) blocks by semi-quantitative scoring by two independent investigators. Multivariable Cox regression models were used to study the associations between miR-24-1-5p expression and prostate cancer recurrence. The prostate cancer patients had a median age of 65.0 years (range 47–75 years). The Cancer of the Prostate Risk Assessment Postsurgical Score, International Society of Urological Pathology grade group, and European Association of Urology Risk group were all significant prognostic factors for five-year recurrence-free survival (p < 0.001). Prostate cancer patients with a high miR-24-1-5p expression (≥1.57) in the tissue had a doubled risk of recurrence compared to patients with low expression (HR 1.99, 95% CI 1.13–3.51). Our study suggests that a high expression of miR-24-1-5p is associated with an increased risk of recurrence of prostate cancer after radical prostatectomy, which points to the potential diagnostic and therapeutic value of detecting miR-24-1-5p in prostate cancer cases.
Highlights
Prostate cancer (PCa) is a major cause of health loss and death worldwide, and it is a heterogeneous disease [1,2]
The prostate cancer patients had an average Body mass Index (BMI) of 27.1 kg/m2, systolic Blood pressure (BP) of 134.9 mmHg (SD 16.8), and diastolic BP of 80.4 mmHg (SD 9.4) at study entry
High miR-24 expression in the tissue (TE + normal epithelial cells (NE)) was associated with an almost doubled risk of the recurrence of prostate cancer compared to that with low miR-24-1-5p expression (HR 1.99, 95% CI 1.13–3.51)
Summary
Prostate cancer (PCa) is a major cause of health loss and death worldwide, and it is a heterogeneous disease [1,2]. We need valid prognostic biomarkers to distinguish low-risk indolent PCa from aggressive PCa. MicroRNAs (miRNAs) are a class of endogenous non-coding small RNA molecules associated with the regulation of gene expression and are “fine-tuners” of the immune system [3]. MicroRNAs (miRNAs) are a class of endogenous non-coding small RNA molecules associated with the regulation of gene expression and are “fine-tuners” of the immune system [3] These have been studied for their potential to serve as molecular prognostic biomarkers for cancer including PCa [4]. In a recent systematic review, fifteen miRNAs were associated with PCa prognosis [4] These are transcribed as ~70 nucleotide precursors in a stemloop sequence and are subsequently processed by the Dicer enzyme to give two mature
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