Expression of microRNAs during female inflorescence development in African oil palm (Elaeis guineensis Jacq.)

Abstract

In African oil palm, the formation of fruit relies on the successful progression of a 2- to 3-year phase of development of inflorescences, in particular the female inflorescence. In this study, we investigated microRNA expression in female inflorescences at two stages of floral development corresponding to the emergence of floral meristems and to the formation of floral organs. High-throughput sequencing data from messenger RNA (mRNA), small RNA, and RNA degradome libraries were used to predict and quantify orthologous and oil palm-specific microRNAs (miRNAs) and their targets. The expression of selected miRNA candidates was validated by quantitative RT-PCR. From female inflorescences, we assembled a reference transcriptome, which allowed us to identify the miRNA precursor sequences and the putative mRNA targets in oil palm. As validated by degradome analysis, we confirmed the cleavage patterns of mRNA targets for oil palm miRNAs. We report here differential gene expression patterns of 18 orthologous miRNA families and their targets in oil palm female inflorescences. Of these, we identified two distinct subsets of orthologous miRNAs that showed inverse expression patterns in female inflorescence of oil palm. We also predicted 15 putative oil palm-specific miRNAs, of which three were validated using quantitative RT-PCR. In oil palm, distinct subsets of miRNAs were differentially expressed at the stage when the floral meristems emerge and at the stage when the floral organs form. These miRNAs are likely to act in concert with their mRNA targets to regulate the early phase of floral organ establishment.