Abstract
MicroRNAs are a class of noncoding RNAs which regulate multiple cellular processes during tumor development. The purpose of this report is to investigate the clinicopathological and prognostic significance of miR-218 in human gliomas. Quantitative RT-PCR (qRT-PCR) was conducted to detect the expression of miR-218 in primary normal human astrocytes, three glioma cell lines and 98 paired glioma and adjacent normal brain tissues.Associations of miR-218 with clinicopathological variables of glioma patients were statistically analyzed. Finally, a survival analysis was performed using the Kaplan-Meier method and Cox's proportional hazards model. The expression level of miR-218 in primary normal human astrocytes was significantly higher than that in glioma cell lines (p<0.01). Also, the expression level of miR-218 in glioma tissues was significantly downregulated in comparison with that in the adjacent normal brain tissues (p<0.001). Statistical analyses demonstrated that low miR-218 expression was closely associated with advanced WHO grade (p=0.002) and low Karnofsky performance score (p=0.010) of glioma patients. Kaplan-Meier analysis with the log-rank test showed that patients with low-miR-218 expression had poorer disease-free survival and overall survival (p=0.0045 and 0.0124, respectively). Multivariate analysis revealed that miR-218 expression was independently associated with the disease-free survival (p=0.009) and overall survival (p=0.004) of glioma patients. Our results indicate that miR-218 is downregulated in gliomas and that its status might be a potential valuable biomarker for glioma patients.
Highlights
Gliomas are the most common and malignant tumors in the brain of humans, which represent about 70% of all brain tumors (Jemal et al, 2011)
QRT-PCR was used to determine the expression of miR-218 in a primary normal human astrocytes (NHA) and three glioma cell lines (U87, U118, T98) normalized to RNU6B
It was observed that the expression level of miR-218 in NHA was significantly higher than that in glioma cell lines (Figure 1A)
Summary
Gliomas are the most common and malignant tumors in the brain of humans, which represent about 70% of all brain tumors (Jemal et al, 2011). MicroRNA-218 (miR-218) has been reported to serve as a tumor suppressor in numerous types of cancer by regulation of the expression of target genes. MiR-218 could inhibit glioma invasion, migration, proliferation, and cancer stem-like cell selfrenewal by targeting the polycomb group gene Bmi (Tu et al, 2013). Materials and Methods: Quantitative RT-PCR (qRT-PCR) was conducted to detect the expression of miR-218 in primary normal human astrocytes, three glioma cell lines and 98 paired glioma and adjacent normal brain tissues.Associations of miR-218 with clinicopathological variables of glioma patients were statistically analyzed. Results: The expression level of miR-218 in primary normal human astrocytes was significantly higher than that in glioma cell lines (p
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