Expression of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinase-1 in Carotid Stenosis

Abstract

It is still controversial whether the specific subtypes of matrix metalloproteinases are related to clinical characteristics in carotid stenosis patients. Carotid atherosclerotic plaques were obtained from 34 patients, 21 males and 13 females with mean age of 71.1. Carotid endarterectomy was performed under the monitoring of EEG and carotid stump pressure. Expression of matrix metalloproteinase-2, 8, 9, and tissue inhibitor of metalloproteinase-1 in the atherosclerotic plaque was assessed by immunohistochemical slides stained with primary antibodies semi-quantitatively. Twenty patients (58.8%) were symptomatic stenosis and 15 (44.1%) had stable plaques without evidence of fibrous cap rupture. Strong stain (degree 3) of matrix metalloproteinase-2 and 9 was dominant in asymptomatic group (P= 0.026 and P= 0.0007, respectively). Strong stain of matrix metalloproteinase-2 was dominant in stable plaque group (P= 0.026). Specific subtypes of matrix metalloproteinases can be related to clinical characteristics such as symptoms or plaque morphology.