Expression of magnesium transporter SLC41A1 in the striatum of 6-hydroxydopamine-induced parkinsonian rats

Abstract

ObjectivesThe aim of this study was to investigate the expression of the mammalian Mg2+ transporter solute carrier family 41, member 1 (SLC41A1) in the striatum (STR) of a 6-hydroxydopamine (6-OHDA)-induced rat model, and its response to magnesium before the degenerative process commenced. Materials and methodsA unilateral parkinsonian rat model was induced by injection of 6-OHDA into the right medial forebrain bundle. Some rats received MgSO4 (90 mg/kg/day, intraperitoneal injection) for 7 or 14 days starting from the day following the 6-OHDA injection. The extent of dopamine depletion was determined by assessing the numbers of tyrosine hydroxylase (TH)-immunoreactive neurons in the substantia nigra pars compacta (SNc) and the apomorphine (APO)-induced rotational behavior. The mRNA and protein expression of SLC41A1 in STR were evaluated by real-time RT-PCR and western blotting respectively. ResultsAPO-induced rotations increased significantly in the 6-OHDA lesioned rats compared with the controls, with MgSO4 administration significantly improving the behavior. The numbers of TH-immunoreactive neurons in SNc were significantly lower in the lesioned side than in the unlesioned side. Administration of MgSO4 for 14 days partly ameliorated the loss of TH-positive neurons. The mRNA and protein expressions of SLC41A1 in the lesioned STR were lower in the 6-OHDA lesioned rats than in the controls at both 7 and 14 days post-lesion induction. MgSO4 supplementation partly reversed the mRNA and protein expressions of SLC41A1. ConclusionThe regulation of SLC41A1 expression is responsive to magnesium in a 6-OHDA-induced rat model, wherein 6-OHDA may alter magnesium transport in the brain.