Abstract
71 patients with gastric cancer in Fifth Hospital in Wuhan from June 2014 to March 2018 were selected as research subjects. Western blot was used to detect the expression of MACC1 in gastric cancer tissue and normal gastric mucosa tissue, and gastric cancer cell SGC7901 was transfected. Transfection group (transfected with MACC1-siRNA), negative control group (transfected with siRNA-NC) and blank control group (untreated cells) were set up. After transfection, the expressions of MACC1 protein and mRNA in the 3 groups were detected by Western blot and qRT-PCR methods, the cell proliferation was detected by MTT method, and the invasion ability of cells in vitro was detected by Transwell chamber. The expression of MACC1 protein in gastric cancer tissue was higher than the control group (P< 0.05). The expression of MACC1 protein in gastric cancer was related to the differentiation degree, infiltration depth, lymph node metastasis and different stages of gastric cancer (P< 0.05). After transfection, the expressions of MACC1 protein and mRNA in the transfection group was significantly lower than the negative control group and blank group (P< 0.05). There was no significant difference in cell viability between the blank group and negative control group at each time point (P> 0.05). MACC1 was highly expressed in gastric cancer tissues. The expression of MACC1 was related to the differentiation degree, infiltration depth, lymph node metastasis and staging of gastric cancer. Down-regulation of MACC1 could inhibit the proliferation and invasion of gastric cancer cells. This study provided a certain biological basis for early clinical prediction, diagnosis and treatment of gastric cancer.
Highlights
Gastric cancer is known as one of the common malignant tumors with high morbidity and mortality
The expressions of metastasis-associated colon cancer gene 1 (MACC1) protein and mRNA in the 3 groups were detected by Western blot and qRT-PCR methods, the cell proliferation was detected by MTT method, and the invasion ability of cells in vitro was detected by Transwell chamber
The high mortality rate of gastric cancer patients is mainly due to the recurrence and metastasis of tumor caused by the strong apoptosis resistance of gastric cancer cells [4], and this process is closely related to abnormal gene expression, cell proliferation, invasion and migration [5]
Summary
Gastric cancer is known as one of the common malignant tumors with high morbidity and mortality. It has been controlled in morbidity and prognosis in recent years, but the development trend of gastric cancer patients is still not optimistic [1]. The high mortality rate of gastric cancer patients is mainly due to the recurrence and metastasis of tumor caused by the strong apoptosis resistance of gastric cancer cells [4], and this process is closely related to abnormal gene expression, cell proliferation, invasion and migration [5].
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