Expression of long non-coding RNA in renal clear cell carcinoma

Abstract

Objective To analyze the expression of long non-coding RNA (lncRNA) in renal clear cell carcinoma (RCCC), the association of lncRNA with RCCC, as well as the role of lncRNA in the diagnosis and treatment of RCCC. Methods Forty fresh RCCC tissues and their normal adjacent tissues were collected from March 2012 to June 2013, and total RNA was extracted using Trizol reagents, purified and tested by denaturing agarose gel electrophmesis and NanoDrop 1000. Through Arraystar Human LncRNA Microarray, the different expression of lncRNA between RCCC and normal adjacent tissues was screened. RT-qPCR was used to verify the expression of lncRNA in 40 pair RCCC tissues and normal adjacent tissues. The receiver-operating characteristic (ROC) curve was adopted to verify the diagnostic efficiency of the selected lncRNA. Results LncRNA expression profile showed 1 787 lncRNA with expression alteration in two fold or above, up-regulated and down-regulated candidate lncRNAs were 941 and 846 respectively. Compared with the adjacent tissues, NR_034095 and NR_038974 were up-regulated in RCCC, and ENS0571724 and ENS0566575 were down-regulated, which were consistent with the microarray analysis. By the ROC curves of NR_034095, NR_038974, ENS0571724 and ENS0566575 to discriminate the RCCC from normal adjacent tissue, the area under curve was 0.928 (95%CI 0.873-0.984), 0.759 (95%CI 0.647-0.871), 0.833 (95%CI 0.747-0.919) and 0.887 (95%CI 0.815-0.959), respectively. Conclusions NR_034095, NR_038974, ENS0571724 and ENS0566575 are significantly differently expressed in RCCC. The different expressed lncRNA might be closely related to the process of RCCC, and may be used as a new candidate target for molecular diagnosis and gene therapy of RCCC. Key words: Carcinoma, renal cell; Adenocarcinoma, clear cell; Long non-coding RNA; Gene expression profiling