Expression of lncRNA-ANRIL before and after Treatment and Its Predictive Value for Short-Term Survival in Patients with Coronary Heart Disease.

Jinhui Sun,Shi Qiu

doi:10.1155/2021/5431985

Abstract

This study aimed at observing the expression of lncRNA-ANRIL (ANRIL) before and after treatment and its predictive value for short-term survival in patients with coronary heart disease (CHD). Altogether, 112 patients with CHD admitted to the hospital were enrolled as a study group (SG), which was divided into a pretreatment study group (preSG) and a posttreatment study group (postSG). Further 72 healthy people undergoing physical examinations during the same period were enrolled as a control group (CG). Peripheral blood was collected from the subjects in the three groups, to detect the expression level of serum ANRIL using quantitative reverse transcription PCR (qRT-PCR). A receiver operating characteristic (ROC) curve was plotted to evaluate the diagnostic value of ANRIL for CHD. Kaplan-Meier survival curves were plotted to analyze 3-year survival rates in high- and low-ANRIL expression groups. Cox regression was conducted to analyze independent risk factors affecting the patients. The expression level of serum ANRIL in preSG was significantly lower than those in CG and postSG (P < 0.05). According to the ROC curve, the area under the curve (AUC) of serum ANRIL for diagnosing CHD in CG was 0.894 and the optimal cutoff value was 0.639, with the sensitivity of 86.61% and the specificity of 93.67%. According to the survival curves, the 3-year overall survival rate in the high-ANRIL expression group was significantly lower than that in the low-expression group (P < 0.05). History of smoking, high total cholesterol (TC), high triglyceride (TG), high homocysteine (Hcy), and ANRIL expression were independent prognostic factors affecting the overall survival time of the patients (P < 0.05). ANRIL is poorly expressed in the peripheral blood of patients with CHD. Its detection has good sensitivity and specificity for diagnosing the disease, and its expression may be related to the poor prognosis of the patients.

Highlights

Coronary heart disease (CHD) is a major cause of patient death in developing countries [1] and a main reason for diseases and disabilities [2, 3]
A total of 112 patients with coronary heart disease (CHD) admitted to the Department of Cardiology in The Second Hospital of Shandong University from May 2015 to February 2016 were enrolled as the study group (SG), which was divided into a pretreatment study group and a posttreatment study group
There were no significant differences between SG and control group (CG) in gender, age, body mass index (BMI), course of disease, place of residence, Table 2: Relationship between clinicopathological parameters and relative expression of ANRIL in peripheral blood (−x ± sd)

Summary

Introduction

Coronary heart disease (CHD) is a major cause of patient death in developing countries [1] and a main reason for diseases and disabilities [2, 3]. CHD has an increasing incidence with the aging of the population [7] and changing disease conditions. Long noncoding RNAs (lncRNAs), as a new type of noncoding RNA, play a vital role in chromatin modification, cell differentiation and proliferation, translation, transcription, and other biological processes [8,9,10]. Many disordered lncRNAs that are found in various tumors can regulate gene transcription and play an important role in tumorigenesis and they can act as tumor suppressor genes or oncogenes [11]. Increasing evidences show that lncRNAs play vital roles in regulating the apoptosis of myocardial cells in myocardial ischemia/reperfusion injury [12,13,14]. Long non-coding RNA antisense noncoding RNA at the INK4 locus (ANRIL), known as Categories Gender

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Conclusion