Expression of Lewis y antigen and integrin αv, β3 in ovarian cancer and their relationship with chemotherapeutic drug resistance

Jian Gao,Juanjuan Liu,Chuan Liu,Dawo Liu,Song Gao,Bei Lin,Shulan Zhang,Rui Hou,Zhenhua Hu,Danye Zhang

doi:10.1186/1756-9966-32-36

Jian Gao, Juanjuan Liu + Show 8 more

Open Access

https://doi.org/10.1186/1756-9966-32-36

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Abstract

ObjectiveThis study investigates the expression of Lewis y antigen, integrin αv, β3 in epithelial ovarian cancer tissues. We further evaluate the relationship between their expression and chemotherapy resistance of ovarian cancer and its possible clinical significance.MethodsTissues of 92 patients with ovarian cancer meeting the inclusion criteria with complete follow-up data were enrolled and divided into chemotherapy resistant group and sensitive group. The expression and relationship of Lewis y antigen and integrin αv, β3 are assessed in paraffin sections using immunohistochemistry and double-labeling immunofluorescence method. Multivariate logistic regression analysis was used to investigate the relationship between age, clinical stage, differentiation, histologic subtype, Lewis y antigen and integrin αv, β3 expression in ovarian cancer patients.ResultsThe expression rates of Lewis y antigen and integrin αv in the resistant group, significantly higher than the rates found in the sensitive group (p <0.05). Multivariate analysis showed that the expression of Lewis y antigen, integrin αv and ovarian cancer’s clinical stage were independent, drug resistance-related risk factors. The expression levels of Lewis y antigen and integrin αv, β3 were positively correlated with each other.ConclusionsA close correlation between Lewis y antigen, integrin αv, β3 and ovarian cancer was observed. Lewis y antigen can influence the biological behavior of a tumor cell as an important composition of integrin αv, β3 by some signal pathway. And the expression of Lewis y antigen, integrin αv and ovarian cancer’s clinical stage are both independent, drug resistance-related risk factors.

Highlights

Ovarian cancer has the highest mortality rate of all cancers of the female reproductive system
We have found that as a part of the integrin αvβ3 structure, Lewis y antigen expression is related to the degree of invasiveness of ovarian cancer [6]
A colon cancer sample served as positive control for Lewis y antigen, and a breast cancer sample was a positive control for integrin αv, β3

Summary

Introduction

Ovarian cancer has the highest mortality rate of all cancers of the female reproductive system. Chemotherapy resistance is an important factor influencing treatment efficacy. Studies have shown that through the interaction between surface adhesion molecules and surrounding extracellular matrix, tumor cells can promote proliferation, invasion, and metastasis, improving their tolerance to chemotherapeutic drugs [1]. Cell adhesion-mediated drug resistance (CAM-DR) is a relatively new theory for the mechanism of drug of Lewis y antigen and integrin αvβ in tissue specimens from patients with chemotherapy resistant or sensitive ovarian cancer and analyze how the expression of these molecules correlates with chemotherapy resistance and the resulting clinical significance

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Conclusion