Abstract
Ca2+ signals regulate many cellular functions, including proliferation. They are governed by the inositol 1,4,5-trisphosphate receptor (IP3R), the only intracellular hepatic Ca2+ channel and by the endoplasmic reticulum Ca2+ pumps, SERCA. To characterise their role in regeneration, expression of their isoforms was studied after 2/3 hepatectomy by real-time quantitative PCR, Western blot and binding studies. We found an early increase in the expression of the IP3R isoform 1 which contrasted with the decrease of the expression of the IP3R isoforms 2 and 3 and of SERCA3. This results in a transient switch between IP3R isoforms 1 and 2, IP3R isoform 1 becoming predominant before the first round of mitosis. Binding studies detected a 30% diminution of the IP3R population at 24 h. In conclusion, the Ca2+ signalling machinery is regulated, after hepatectomy, by changes in expression of the IP3R and SERCA isoforms to adapt Ca2+ signals to the regenerative state.
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